1x evagreen (Jena Bioscience)
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1x evagreen
1x Evagreen, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/evagreen+fluorescent+dna+stain/InfiniteGreen+Fluorescent+DNA+Stain/bio_rxiv__64898__2026__05__06__723196-362-21-23
Average 94 stars, based on 14 article reviews
1x Evagreen, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/evagreen+fluorescent+dna+stain/InfiniteGreen+Fluorescent+DNA+Stain/bio_rxiv__64898__2026__05__06__723196-362-21-23
Average 94 stars, based on 14 article reviews
1x evagreen - by Bioz Stars,
2026-10
94/100 stars
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Gene Expression:Article Title: Deazaflavin metabolite produced by endosymbiotic bacteria controls fungal host reproduction Article Snippet: .. All primer pairs have an efficiency of 93% to 96% and were used in subsequent gene expression experiments ( ) using My Taq HS Mix (Bioline, London, UK) and Staining:Article Title: Deazaflavin metabolite produced by endosymbiotic bacteria controls fungal host reproduction Article Snippet: .. All primer pairs have an efficiency of 93% to 96% and were used in subsequent gene expression experiments ( ) using My Taq HS Mix (Bioline, London, UK) and Article Title: Microcapsule for detecting and/or quantitating an analyte in a sample Article Snippet: Components Per mL Polymerisation Mix Reaction mix 20% (w/v) N-Isopropylacrylamide (NIPAM) 250 μl 2.5% (w/v) N,N′-Methylenebisacrylamide 20 μl 2.5% Biotin-modified monomer 10 μl 5% (w/v) Ammonium persulfate 150 μl Deionised water 570 μl Component 2 (Reagent Phase): A (2-Hydroxypropyl)-y-cyclodextrine stock solution (100%) was also prepared in nuclease-free water using Cavasol W7 or W8 (Sigma) and stored at room temperature. .. The final mixture for PCR accommodating all reagents to be encapsulated contained the following: 1.25 U/μl Hot Start Taq DNA Polymerase (biotechrabbit GmbH) 4.0 mM dNTPs (biotechrabbit GmbH) 8.0 μM sense primer (5′-GCAGTGGCGCCCGAACAGG-3′) (Metabion International AG) 8.0 μM antisense primer (5′-ACTGACGCTCTCGCACCCATCT-3′) (Metabion International AG) 8.0 μM Taq-Man probe (5′-CF647-CTCCGACGCAACGGGCTCG-BHQ3-3′) (Metabion International AG) or 10× Article Title: Stimulus-responsive nanocarrier from star-shaped polyethyleneimine-β-cyclodextrin coated mesoporous silica for targeted combination cancer therapy Article Snippet: In this study, a stimulus-responsive organic/inorganic supramolecular nanostructure (SRN) was designed to develop a multifunctional nano-drug delivery system for combined chemo/gene/magnetic hyperthermia therapy targeting multidrug-resistant cancer.. SRNs have been successfully fabricated based on host/guest interactions between folate-appended-polyethyleneimine-β-cyclodextrin (FPC) and benzimidazole-modified magnetic-hollow mesoporous silica nanoparticles (BM-MHMSNs).. A typical antitumor drug, Doxorubicin (DOX), was successfully loaded in the pore of BM-MHMSNs and capped by FPC. Article Title: Dynamics of the milk microbial community during subacute ruminal acidosis with or without intramammary lipopolysaccharide challenge in dairy cows Article Snippet: .. The DNA samples were assayed in duplicate in a 23 μL reaction mixture, which contained 12.7 μL DEPC-treated water, 2.5 μL 10 × buffer, 1.0 μL 2 mM MgCl 2 (stock concentration 50 mM), 2.5 μL of each primer (stock concentration 2.5 μM), 0.5 μL of Article Title: Microcapsule for detecting and/or quantitating an analyte in a sample Article Snippet: A (2-Hydroxypropyl)-y-cyclodextrine stock solution (100%) was also prepared in nuclease-free water using Cavasol W7 or W8 (Sigma) and stored at room temperature. .. The final mixture for PCR accommodating all reagents to be encapsulated contained the following: Component 2: 1.25 U/μl Hot Start Taq DNA Polymerase (biotechrabbit GmbH) 4.0 mM dNTPs (biotechrabbit GmbH) 8.0 μM sense primer (5′-GCAGTGGCGCCCGAACAGG-3′) (Metabion International AG) 8.0 μM antisense primer (5′-ACTGACGCTCTCGCACCCATCT-3′) (Metabion International AG) 8.0 μM Taq-Man probe (5′-Cy5-CTCCGACGCAACGGGCTCG-BHQ3-3′) (Metabion International AG) or 10× Article Title: Mouse nuclear RNAi-defective 2 promotes splicing of weak 5' splice sites. Article Snippet: The reaction was slowly cooled down to room temperature with a Δ −1°C/sec gradient, and 0.5 μL each of 5 U/μL RNase H (NEB), 1000U/μLRNase T1 (ThermoFisher Scientific), and 20U/μLExonuclease I (NEB)were added todegrade the excess TSOandRTprimer for 20minat37°C, followedbya15-minheat inactivationat 80°C. .. To amplify the cDNA library, the eluates from the previous step were transferred to a 0.2-mL low-profile PCR tube (Bio-Rad) and mixed with a master mix consisting of 20 μL of 2× NEBNext Ultra II Q5 Master Mix (NEB), 1 μL of 40 μM P5∗ primer, 1 μL of 40 μM PE∗ primer, and 0.8 μL of 100 μM Article Title: Dynamics of the milk microbial community during subacute ruminal acidosis with or without intramammary lipopolysaccharide challenge in dairy cows. Article Snippet: The resulting DNA was purified using the innuPREP PCRpure Kit (ist Innuscreen GmbH, Berlin, Germany) and quantified with the Qubit ds BR Assay Kit (Thermo Fisher Scientific, Austria). .. The DNA samples were assayed in duplicate in a 23 μL reaction mixture, which contained 12.7 μL DEPC-treated water, 2.5 μL 10 × buffer, 1.0 μL 2 mM MgCl2 (stock concentration 50 mM), 2.5 μL of each primer (stock concentration 2.5 μM), 0.5 μL of Polymerase Chain Reaction:Article Title: Microcapsule for detecting and/or quantitating an analyte in a sample Article Snippet: Components Per mL Polymerisation Mix Reaction mix 20% (w/v) N-Isopropylacrylamide (NIPAM) 250 μl 2.5% (w/v) N,N′-Methylenebisacrylamide 20 μl 2.5% Biotin-modified monomer 10 μl 5% (w/v) Ammonium persulfate 150 μl Deionised water 570 μl Component 2 (Reagent Phase): A (2-Hydroxypropyl)-y-cyclodextrine stock solution (100%) was also prepared in nuclease-free water using Cavasol W7 or W8 (Sigma) and stored at room temperature. .. 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The DNA samples were assayed in duplicate in a 23 μL reaction mixture, which contained 12.7 μL DEPC-treated water, 2.5 μL 10 × buffer, 1.0 μL 2 mM MgCl 2 (stock concentration 50 mM), 2.5 μL of each primer (stock concentration 2.5 μM), 0.5 μL of Article Title: Dynamics of the milk microbial community during subacute ruminal acidosis with or without intramammary lipopolysaccharide challenge in dairy cows. Article Snippet: The resulting DNA was purified using the innuPREP PCRpure Kit (ist Innuscreen GmbH, Berlin, Germany) and quantified with the Qubit ds BR Assay Kit (Thermo Fisher Scientific, Austria). .. The DNA samples were assayed in duplicate in a 23 μL reaction mixture, which contained 12.7 μL DEPC-treated water, 2.5 μL 10 × buffer, 1.0 μL 2 mM MgCl2 (stock concentration 50 mM), 2.5 μL of each primer (stock concentration 2.5 μM), 0.5 μL of Platinum:Article Title: Dynamics of the milk microbial community during subacute ruminal acidosis with or without intramammary lipopolysaccharide challenge in dairy cows Article Snippet: .. The DNA samples were assayed in duplicate in a 23 μL reaction mixture, which contained 12.7 μL DEPC-treated water, 2.5 μL 10 × buffer, 1.0 μL 2 mM MgCl 2 (stock concentration 50 mM), 2.5 μL of each primer (stock concentration 2.5 μM), 0.5 μL of cDNA Library Assay:Article Title: Mouse nuclear RNAi-defective 2 promotes splicing of weak 5' splice sites. Article Snippet: The reaction was slowly cooled down to room temperature with a Δ −1°C/sec gradient, and 0.5 μL each of 5 U/μL RNase H (NEB), 1000U/μLRNase T1 (ThermoFisher Scientific), and 20U/μLExonuclease I (NEB)were added todegrade the excess TSOandRTprimer for 20minat37°C, followedbya15-minheat inactivationat 80°C. .. To amplify the cDNA library, the eluates from the previous step were transferred to a 0.2-mL low-profile PCR tube (Bio-Rad) and mixed with a master mix consisting of 20 μL of 2× NEBNext Ultra II Q5 Master Mix (NEB), 1 μL of 40 μM P5∗ primer, 1 μL of 40 μM PE∗ primer, and 0.8 μL of 100 μM |
